Affiliation:
1. Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520
Abstract
ABSTRACT
A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon’s site of insertion has been developed. The basic plasposon, named Tn
Mod
, consists of the Tn
5
inverted repeats, a conditional origin of replication, rare restriction endonuclease multiple cloning sites, and exchangeable antibiotic resistance cassettes. The broad host range and low target DNA sequence specificity of the Tn
5
transposase, in combination with the flexibility afforded by the modular arrangement of Tn
Mod
, result in a versatile tool for the mapping of insertional mutations and the rapid recovery of clones from gram-negative bacteria.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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