Dialysis membrane technique for ultrastructural studies of microbial interactions

Abstract

A dialysis membrane technique was developed that enabled ultrastructural investigations of the interaction of nematode-trapping fungi and their nematode prey. It allowed the sectioning of individual traps that had been selected by light microscopy and was used in kinetic studies on trap formation, nematode capture, and subsequent nematode digestion. The method can also be used for enzyme cytochemical experiments.