Affiliation:
1. Department of Microbiology, Georgetown University Schools of Medicine and Dentistry, Washington, D. C. 20007
Abstract
A chemically defined medium, consisting of
d
-fructose,
l
-glutamic acid,
l
-histidine, K
2
HPO
4
, MgSO
4
·7H
2
O, ZnSO
4
·7H
2
O, CaCl
2
·2H
2
O, FeSO
4
·7H
2
O, CoCl
2
·6H
2
O, and deionized water, was developed for synthesis of high yields (500 to 600 μg/ml) of actinomycin D by
Streptomyces parvulus
. Under these nutritional conditions, growth and actinomycin formation did not follow a typical trophophase-idiophase pattern. The amino acids appeared to have a sparing action on the utilization of
d
-fructose which was slowly and incompletely metabolized during mycelium development and antibiotic production. A significant repression of actinomycin synthesis by
S. parvulus
was observed when
d
-glucose (0.01 to 0.25%) was added to the culture medium. The repression was not due to a decline in the pH of the medium during glucose catabolism.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference60 articles.
1. Some nutritional requirements of Streptomyces griseus for growth and candicidin production;Acker R. F.;Appl. Microbiol.,1954
2. Metabolism of proline and of hydroxyproline;Adams E.;Int. Rev. Connect. Tissue Res.,1970
3. Enniatin production by Fusarium sambucinum; primary, secondary and unitary metabolism;Audhya T. K.;J. Gen. Microbiol.,1975
4. Mineral nutrition of Streptomyces kanamyceticus for kanamycin formation;Basek K.;Antimicrob. Agents Chemother.,1975
5. Bernt E. and H. U. Bergmeyer. 1974. is-Fructose p. 1304-1307. In H: U. Bergmeyer (ed.) Methods of enzymatic analysis vol. 3. Academic Press Inc. New York.
Cited by
60 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献