Characterization of the Polymerase and RNase H Activities of Human Foamy Virus Reverse Transcriptase

Author:

Boyer Paul L.1,Stenbak Carolyn R.23,Clark Patrick K.4,Linial Maxine L.23,Hughes Stephen H.1

Affiliation:

1. HIV Drug Resistance Program, Center for Cancer Research, National Cancer Institute-Frederick

2. Division of Basic Sciences, Fred Hutchison Cancer Research Center, Seattle, Washington 98109

3. Department of Microbiology, University of Washington, Seattle, Washington 98195

4. Basic Research Program, SAIC-Frederick, Frederick, Maryland 21702

Abstract

ABSTRACT Foamy virus (FV) replication, while related to that of orthoretroviruses, differs at a number of steps. Several of these differences involve the reverse transcriptase (RT). There appear to be fewer RTs present in FV than in orthoretroviruses; we previously proposed that the polymerase of FV RT was more active than orthoretroviral RTs to compensate for the numerical difference. Here we present further characterization of the RT of FV. The polymerase activity of FV RT was greater than that of human immunodeficiency virus type 1 RT in a variety of assays. We also examined the RNase H activity of FV RT, and we propose that FV RT has a basic loop in the RNase H domain. Although the sequence of the basic loop of FV RT is different from the basic loop of either Moloney leukemia virus RNase H or Escherichia coli RNase H, the FV RT basic loop appears to have a similar function.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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