Development of a Multiplex Real-Time PCR for Detection and Differentiation of Malignant Catarrhal Fever Viruses in Clinical Samples-Reference-Cited by-同舟云学术

Development of a Multiplex Real-Time PCR for Detection and Differentiation of Malignant Catarrhal Fever Viruses in Clinical Samples

Published:2009-08 Issue:8 Volume:47 Page:2586-2589
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cunha Cristina W.¹,Otto Lisa²,Taus Naomi S.¹,Knowles Donald P.¹²,Li Hong¹

Affiliation:

1. Animal Disease Research Unit, USDA-ARS, Washington State University, P.O. Box 646630, Pullman, Washington 99164-6630

2. College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7040

Abstract

ABSTRACT A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00997-09

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