Affiliation:
1. Institute of Biotechnology I, Research Center Juelich, Juelich, Germany
2. Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Muenster, Germany
Abstract
ABSTRACT
The amino acid-producing organism
Corynebacterium glutamicum
cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of
Escherichia coli
glycerol utilization genes,
C. glutamicum
was engineered to grow on glycerol. While expression of the
E. coli
genes for glycerol kinase (
glpK
) and glycerol 3-phosphate dehydrogenase (
glpD
) was sufficient for growth on glycerol as the sole carbon and energy source, additional expression of the aquaglyceroporin gene
glpF
from
E. coli
increased growth rate and biomass formation. Glutamate production from glycerol was enabled by plasmid-borne expression of
E. coli glpF, glpK
, and
glpD
in
C. glutamicum
wild type. In addition, a lysine-producing
C. glutamicum
strain expressing
E. coli glpF, glpK
, and
glpD
was able to produce lysine from glycerol as the sole carbon substrate as well as from glycerol-glucose mixtures.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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