Development of Natural Culture Media for Rapid Induction of Fonsecaea pedrosoi Sclerotic Cells In Vitro

Author:

da Silva Moises Batista1,da Silva Jorge Pereira12,Sirleide Pereira Yamano Suellen1,Salgado Ubirajara Imbiriba13,Diniz José Antonio Picanço4,Salgado Claudio Guedes15

Affiliation:

1. Laboratório de Dermato-Imunologia, Universidade do Estado do Pará (UEPA), Universidade Federal do Pará (UFPA), and Unidade de Referência em Dermatologia Sanitária do Estado do Pará Dr. Marcello Candia (MC), Marituba

2. Departamento de Farmácia

3. Serviço de Dermatologia, UEPA

4. Laboratório de Microscopia Eletrônica do Instituto Evandro Chagas, Belém, Pará, Brazil

5. Departamento de Patologia, UFPA

Abstract

ABSTRACT Fonsecaea pedrosoi is the main agent of chromoblastomycosis, a skin disease presenting verrucous lesions, in which round, thick-walled sclerotic cells are found. In vitro induction of sclerotic cells is time-consuming (20 to 45 days) and temperature dependent. We present two new natural media that reduce the sclerotic-cell induction time to only 2 days.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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