Affiliation:
1. Department of Molecular Biology, Cleveland Clinic Foundation, Ohio 44195-5069.
Abstract
Extracts made from human respiratory syncytial virus (RSV)-infected Hep-2 cells synthesized mRNAs encoded by all known viral genes. In contrast, RSV ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected Hep-2 cells, demonstrating that a cellular factor(s) has a role in RSV gene expression. Quantitation of the individual gene mRNAs transcribed in vitro revealed polarity of transcription of the genome.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology