Evidence for circular permutation of the prophage genome of Bacillus subtilis bacteriophage phi 105

Author:

Marrero R,Yasbin R E

Abstract

Analysis of DNA extracted from Bacillus subtilis lysogenic for bacteriophage phi 105 was performed by restriction endonuclease digestion and Southern hybridization using mature phi 105 DNA as a probe. The data revealed that the phi 105 prophage is circularly permuted. Digests using the enzymes EcoRI, SmaI, PstI, and HindIII localized the bacteriophage attachment site (att) to a region 63.4 to 65.7% from the left end of the mature bacteriophage genome. The phi 105 att site-containing SmaI C, PstI J, and HindIII L fragments were not present in digests of phi 105 prophage DNA. phi 105-homologous "junction" fragments were visualized by probing digests of prophage DNA with the purified PstI J fragment isolated from the mature bacteriophage genome. The excision of the phi 105 prophage was detected by observing the appearance of the mature PstI J fragment and the concomitant disappearance of a junction fragment during the course of prophage induction.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference30 articles.

1. Mapping of prophage and mature deoxyribonucleic acid from temperate Bacillus bacteriophage 4105 by marker rescue;Armentrout R. W.;J. Virol.,1970

2. Armentrout R. W. and L. Rutberg. 1971. Heat induction of prophage 4105 in Bacillus subtilis: replication of the bacterial 1148 NOTES

3. Characterization of temperate Bacillus bacteriophage 4105;Birdsell D. C.;J. Virol.,1969

4. Restriction enzyme analysis of Bacillus subtilis bacteriophage 4105 DNA;Bugaichuk U. D.;J. Gen. Microbiol.,1984

5. Campbell A. 1983. Bacteriophage X p. 65-103. In J. A. Shapiro (ed.) Mobile genetic elements Academic Press Inc. New York.

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Temperate Phage Vectors;Bacillus subtilis and Other Gram-Positive Bacteria;2014-04-30

2. Identification of Bacillus subtilis genes for septum placement and shape determination;Journal of Bacteriology;1992-11

3. Temperate Bacteriophages of Bacillus subtilis;The Bacteriophages;1988

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3