Development of an enzyme-linked immunosorbent assay method for typing and quantitation of Klebsiella pneumoniae lipopolysaccharide: application to serotype O1-Reference-Cited by-同舟云学术

Development of an enzyme-linked immunosorbent assay method for typing and quantitation of Klebsiella pneumoniae lipopolysaccharide: application to serotype O1

Published:1993-05 Issue:5 Volume:31 Page:1379-1381
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Albertí S¹,Hernández-Allés S¹,Gil J¹,Reina J¹,Martínez-Beltrán J¹,Camprubí S¹,Tomás J M¹,Benedí V J¹

Affiliation:

1. Universidad de las Islas Baleares, Palma de Mallorca, Spain.

Abstract

We describe a method for the typing and quantitation of Klebsiella pneumoniae serotype O1 lipopolysaccharide (LPS) based on inhibition in an enzyme-linked immunosorbent assay of a reaction of known O1 LPS antigen and anti-O1 antibody by unknown LPS extracts. Serotype O1 was found in 32% of the 124 K. pneumoniae clinical isolates tested, showing that this serotype is frequent among the eight O serotypes which have been described previously.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.31.5.1379-1381.1993

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