Affiliation:
1. Department of Microbiology, University of Texas, Austin 78712-1095.
Abstract
The endogenous Mtv-8 provirus previously has been mapped within approximately 0.52 centimorgan from several V kappa markers on mouse chromosome 6. Using Southern blotting and DNA from a recombinant backcross mouse from the C57BL/6 (Mtv-8 positive) and C58 (Mtv-8 negative) strains, Mtv-8 was localized to the same side of the crossover point as immunoglobulin kappa (Ig kappa)-V24 but on the opposite side of the crossover from Ig kappa-V10 and Ig kappa-V21. Molecular cloning and characterization of cellular DNA adjacent to Mtv-8 revealed a functional V kappa 9 gene approximately 4.6 kb downstream and in the same transcriptional orientation as the provirus. These data suggest that Mtv-8 is within the centromere-proximal portion of the V kappa locus.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference27 articles.
1. A linkage map of mouse chromosome 12: localization of Igh and effects of sex and interference on recombination;Blank R. D.;Genetics,1988
2. Two genetically transmitted BALB/c mouse mammary tumor virus genomes located on chromosomes 12 and 16;Callahan R.;J. Virol.,1984
3. Sets of immunoglobulin VK genes homologous to ten cloned VK sequences: implications for the number of germline VK genes;Cory S.;J. Mol. Appl. Genet.,1981
4. The organization of immunoglobulin variable kappa chain genes on mouse chromosome 6;D'Hoostelaere L. A.;Immunogenetics,1986
5. The IgK L chain allelic groups among the IgK haplotypes and IgK crossover populations suggest a gene order;D'Hoostelaere L. A.;J. Immunol.,1988
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献