Analysis of 1,25-Dihydroxyvitamin D 3 Genomic Action Reveals Calcium-Regulating and Calcium-Independent Effects in Mouse Intestine and Human Enteroids-Reference-Cited by-同舟云学术

Analysis of 1,25-Dihydroxyvitamin D 3 Genomic Action Reveals Calcium-Regulating and Calcium-Independent Effects in Mouse Intestine and Human Enteroids

Published:2020-12-21 Issue:1 Volume:41 Page:
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Li Shanshan¹,De La Cruz Jessica¹,Hutchens Steven²,Mukhopadhyay Somshuvra²,Criss Zachary K.³,Aita Rohit⁴,Pellon-Cardenas Oscar⁴,Hur Joseph⁴,Soteropoulos Patricia¹⁵,Husain Seema¹⁵,Dhawan Puneet¹⁵,Verlinden Lieve⁶,Carmeliet Geert⁶,Fleet James C.⁷,Shroyer Noah F.³,Verzi Michael P.⁴,Christakos Sylvia¹^ORCID

Affiliation:

1. Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers, The State University of New Jersey, New Jersey Medical School, Newark, New Jersey, USA

2. Division of Pharmacology and Toxicology, College of Pharmacy, Institute for Cellular and Molecular Biology and Institute for Neuroscience, The University of Texas at Austin, Austin, Texas, USA

3. Integrative Molecular and Biomedical Sciences Graduate Program, Division of Medicine, Baylor College of Medicine, Houston, Texas, USA

4. Department of Genetics, Rutgers University, New Brunswick, New Jersey, USA

5. Genomics Center, Rutgers, The State University of New Jersey, New Jersey Medical School, Newark, New Jersey, USA

6. Laboratory of Clinical and Experimental Endocrinology, Department of Chronic Diseases and Metabolism, Leuven, Belgium

7. Department of Nutrition Science, Purdue University, West Lafayette, Indiana, USA

Abstract

Although vitamin D is critical for the function of the intestine, most studies have focused on the duodenum. We show that transgenic expression of the vitamin D receptor (VDR) only in the distal intestine of VDR null mice (KO/TG mice) results in the normalization of serum calcium and rescue of rickets. Although it had been suggested that calcium transport in the distal intestine involves a paracellular process, we found that the 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ]-activated genes in the proximal intestine associated with active calcium transport ( Trpv6 , S100g , and Atp2b1 ) are also induced by 1,25(OH) 2 D 3 in the distal intestine of KO/TG mice.

Funder

National Institutes of Health

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.00372-20

Reference85 articles.

1. Vitamin D: Metabolism, Molecular Mechanism of Action, and Pleiotropic Effects

2. Genomic Determinants of Vitamin D-Regulated Gene Expression