Multicenter Collaborative Study for Standardization of Candida albicans Genotyping Using a Polymorphic Microsatellite Marker-Reference-Cited by-同舟云学术

Multicenter Collaborative Study for Standardization of Candida albicans Genotyping Using a Polymorphic Microsatellite Marker

Published:2010-07 Issue:7 Volume:48 Page:2578-2581
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Garcia-Hermoso Dea¹,MacCallum Donna M.²,Lott Timothy J.³,Sampaio Paula⁴,Serna Maria-José Buitrago⁵,Grenouillet Fréderic⁶,Klaassen Corné H. W.⁷,Bretagne Stéphane¹⁸

Affiliation:

1. Institut Pasteur, Unité de Mycologie Moléculaire, Centre National de Référence Mycologie et Antifongiques and CNRS URA3012, 75724 Paris Cedex 15, France

2. Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

3. Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia

4. Centro de Biologia Molecular e Ambiental, Universidade do Minho, Campus Gualtar, Braga, Portugal

5. Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain

6. Laboratoire de Mycologie—Parasitologie, CHU Jean Minjoz, Besançon, France

7. Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, Netherlands

8. Laboratoire de Parasitologie Mycologie, Hôpital Henri Mondor, Créteil, France

Abstract

ABSTRACT Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00040-10

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