High variance in quantification of <i>Mycobacterium tuberculosis</i> at low bacterial loads and with differentially detectable mycobacteria-Reference-Cited by-同舟云学术

High variance in quantification of Mycobacterium tuberculosis at low bacterial loads and with differentially detectable mycobacteria

Published:2024-07-09 Issue:7 Volume:68 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Walsh Kathleen F.¹²^ORCID,Lee Myung Hee¹,Zainabadi Kayvan¹^ORCID,Vilbrun Stalz Charles³,Jean Juste Marc Antoine³,Joseph Yvetot³,Royal Gertrude³,Saito Kohta⁴,McAulay Kathrine¹,Pape Jean W.¹³,Fitzgerald Daniel¹

Affiliation:

1. Center for Global Health, Weill Cornell Medicine, New York, New York, USA

2. Division of General Internal Medicine, Department of Medicine, Weill Cornell Medicine, New York, New York, USA

3. Department of Medicine, Groupe Haïtien d'Étude du Sarcome de Kaposi et des Infectieuses Opportunistes (GHESKIO), Port-au-Prince, Haiti

4. Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, New York, USA

Abstract

ABSTRACT We examined the correlation between three different methods of Mycobacterium tuberculosis quantification: time to positivity (TTP), log 10 CFU, and an assay to detect differentially detectable M. tuberculosis (DD Mtb) from three different prospective studies. Participants with DD Mtb have significantly more variation in the CFU/TTP correlation than participants with no DD Mtb ( P < 0.001). This may impact the design of early bactericidal activity studies that use TTP as the primary outcome.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/aac.01601-23

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