The Presence of Leukocytes in Ex Vivo Assays Significantly Increases the 50-Percent Inhibitory Concentrations of Artesunate and Chloroquine against Plasmodium vivax and Plasmodium falciparum-Reference-Cited by-同舟云学术

The Presence of Leukocytes in Ex Vivo Assays Significantly Increases the 50-Percent Inhibitory Concentrations of Artesunate and Chloroquine against Plasmodium vivax and Plasmodium falciparum

Published:2011-03 Issue:3 Volume:55 Page:1300-1304
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kaewpongsri S.¹,Sriprawat K.¹,Suwanarusk R.²,Kyle D. E.³,Lek-Uthai U.⁴,Leimanis M.¹,Lwin K. M.¹,Phyo A. P.¹,Zwang J.¹,Russell B.²,Nosten F.¹⁵⁶,Renia L.²

Affiliation:

1. Shoklo Malaria Research Unit, Mae Sod, Tak, Thailand

2. Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), A*STAR, Biopolis, Singapore

3. Department of Global Health, College of Public Health, University of South Florida, Mahidol University, Bangkok, Thailand

4. Department of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Bangkok, Thailand

5. Centre for Clinical Vaccinology & Tropical Medicine, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, United Kingdom

6. Faculty of Tropical Medicine, Mahidol University, Rajvithi Road, Bangkok, Thailand

Abstract

ABSTRACT Plasmodium species ex vivo sensitivity assay protocols differ in the requirement for leukocyte removal before culturing. This study shows that the presence of leukocytes significantly increases the 50% inhibitory concentration (IC 50 ) of P. vivax and P. falciparum to artesunate and chloroquine relative to results with the paired leukocyte-free treatment. Although leukocyte removal is not an essential requirement for the conduct of ex vivo assays, its use has important implications for the interpretation of temporal and spatial antimalarial sensitivity data.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01103-10

Reference29 articles.

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