Carbohydrate Fermentation Patterns of Neisseria meningitidis Determined by a Microtiter Method

Author:

Davies J. A.1,Mitzel J. R.1,Beam W. E.1

Affiliation:

1. Naval Medical Field Research Laboratory, Camp Lejeune, North Carolina 28542

Abstract

A carbohydrate fermentation technique has been developed and compared to the standard fermentation test with cystine-Trypticase-semisolid agar for the identification of Neisseria meningitidis . This new method utilizes Mueller-Hinton broth as a basal substrate and is performed with microtiter methods. By using Mueller-Hinton broth with and without the addition of antibiotics, the method can be adjusted to test the fermentation patterns of all of the Neisseria including N. gonorrhoeae .

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference9 articles.

1. Identification of Neisseria meningitidis carbohydrate fermentation patterns in Mueller-Hinton Broth;Beno D. W.;J. Bacteriol.,1968

2. Blair J. E. E. H. Lennette and J. P. Truant. 1970. Manual of clinical microbiology p. 78. American Society for Microbiology Bethesda Md.

3. Bodily H. L. E. L. Updyke and J. 0. Mason. 1970. Diagnostic procedures for bacterial mycotic and parasitic infections p. 143 296. American Public Health Association Inc. New York.

4. Relationship of serogroups of Neisseria meningitidis. I. Microagglutination, gel diffusion, and slide agglutination studies of meningococcal antisera before and after absorption with RAS-10 strain of meningococci;Devine L. F.;Infec. Immun.,1970

5. Proposed model for screening antimicrobial agents for potential use in eliminating meningococci from the nasopharynx of healthy carriers. Antimicro;Devine L. F.;Ag. Chemother.,1969

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