Affiliation:
1. Departments of Clinical Pathology
2. Department of Clinical Pathology, Seonam University, College of Medicine, Gwangju, Korea
3. Internal Medicine, Chonnam National University Medical School
Abstract
ABSTRACT
Biofilm production has been implicated as a potential virulence factor of some
Candida
species responsible for catheter-related fungemia in patients receiving parenteral nutrition. We therefore compared clinical bloodstream isolates representing seven different
Candida
species to each other and to those from other anatomical sites for the capacity to form biofilms in glucose-containing medium. Potential associations between the capacity to form biofilms and the clinical characteristics of fungemia were also analyzed. Isolates included the following from nonneutropenic patients: 101 bloodstream isolates (35
C. parapsilosis
, 30
C. albicans
, 18
C. tropicalis
, 8
C. glabrata
, and 10 other
Candida
species isolates) and 259 clinical isolates from other body sites (116
C. albicans
, 53
C. glabrata
, 43
C. tropicalis
, 17
C. parapsilosis
, and 30 other
Candida
species isolates). Organisms were grown in Sabouraud dextrose broth (SDB) containing a final concentration of 8% glucose to induce biofilm formation, as published previously. Biofilm production was determined by both visual and spectrophotometric methods. In this medium, biofilm production by
C. albicans
isolates was significantly less frequent (8%) than that by non-
C. albicans Candida
species (61%;
P
< 0.0001). The overall proportion of non-
C. albicans Candida
species isolates from the blood that produced biofilms was significantly higher than that of non-
C. albicans Candida
isolates obtained from other sites (79% versus 52%;
P
= 0.0001). Bloodstream isolates of
C. parapsilosis
alone were significantly more likely to be biofilm positive than were
C. parapsilosis
isolates from other sites (86% versus 47%;
P
= 0.0032). Non-
C. albicans Candida
species, including
C. parapsilosis
, were more likely to be biofilm positive if isolates were derived from patients whose candidemia was central venous catheter (CVC) related (95%;
P
< 0.0001) and was associated with the use of total parenteral nutrition (TPN) (94%;
P
< 0.005). These data suggest that the capacity of
Candida
species isolates to produce biofilms in vitro in glucose-containing SDB may be a reflection of the pathogenic potential of these isolates to cause CVC-related fungemia in patients receiving TPN.
Publisher
American Society for Microbiology
Cited by
290 articles.
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