Affiliation:
1. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
Abstract
ABSTRACT
Sequences corresponding to some of the polymorphic loci previously reported from
Entamoeba histolytica
have been detected in
Entamoeba dispar
. Comparison of nucleotide sequences of two loci between
E. dispar
strain SAW760 and
E. histolytica
strain HM-1:IMSS revealed significant differences in both repeat and flanking regions. The tandem repeat units varied not only in sequence but also in number and arrangement between the two species at both the loci. Using the sequences obtained, primer pairs aimed at amplifying species-specific products were designed and tested on a variety of
E. histolytica
and
E. dispar
samples. Amplification results were in complete agreement with the original species classification in all cases, and the PCR products displayed discernible size and pattern variations among the isolates.
Publisher
American Society for Microbiology
Cited by
54 articles.
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