Differentiation between active and inactive human brucellosis by measuring antiprotein humoral immune responses

Author:

Goldbaum F A1,Rubbi C P1,Wallach J C1,Miguel S E1,Baldi P C1,Fossati C A1

Affiliation:

1. Instituto de Estudios de la Inmunidad Humoral (IDEHU-UBA-CONICET), Buenos Aires, Argentina.

Abstract

A preparation of Brucella abortus cytoplasmic proteins was depleted of lipopolysaccharide (LPS) by immunoadsorption with a monoclonal antibody (MAb), BC68, specific for the O antigen of B. abortus smooth LPS. Two enzyme-linked immunosorbent assay (ELISA) systems were developed and used in this study. The first system includes an LPS-free cytoplasmic protein preparation; the second one was based on antigen capture on MAb BC68. By using these systems, we have demonstrated that 94% (33 of 35) of the brucellosis patients studied showed immunoglobulin G antiprotein response and also that all of the patients showed a strong anti-LPS reactivity. Thirty-six serologically positive individuals with no active infection at the time of examination (SPI) were also included. No immunoglobulin G antibodies against proteins were detected in 34 of them (92%), whereas 31 SPI (86%) showed various degrees of anti-LPS reactivity. The use of the LPS-free protein extract in ELISAs made it possible to establish differential reactivity patterns between active and inactive brucellosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference20 articles.

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4. .Centro Panamericano de Zoonosis-Oficina Panamericana Sanitaria. 1971. Nota Tecnica 2 p. 22. Centro Panamericano de Zoonosis-Oficina Panamericana Sanitaria Buenos Aires.

5. Diaz R. and I. Moriyon. 1989. Laboratory techniques in the diagnosis of human brucellosis p. 73-83. In E. J. Young and M. J. Corbel (ed.) Brucellosis: clinical and laboratory aspects. CRC Press Inc. Boca Raton Fla.

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