Affiliation:
1. Department of Biology and the McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218
Abstract
Sugars transported by a bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) require two soluble proteins: HPr, a low-molecular-weight phosphate-carrier protein, and enzyme I. The structural genes coding for HPr (
ptsH
) and Enzyme I (
ptsI
) are shown to be cotransducible in
Salmonella typhimurium
. The gene order of this region of the
Salmonella
chromosome is
cysA-trzA-ptsH-ptsI
...(
crr
). A method for the isolation of
trzA-pts
deletion is described. One class of
pts
deletions extends through
ptsH
and into
ptsI
; a second class includes both
ptsH
and
ptsI
and extends into or through the
crr
gene. The
crr
gene either codes for or regulates the synthesis of a third PTS protein (factor III) which is sugar-specific. A hypothesis is presented for a mechanism of deletion formation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference19 articles.
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