Affiliation:
1. Centro di Endocrinologia ed Oncologia Sperimentale del CNR, Istituto di Patologia Generale dell'Università, 80138 Naples, Italy, and Istituto Internazionale di Genetica e Biofisica, Via Marconi 10, Naples, Italy
Abstract
In vitro studies have been of great value in elucidating the mechanism of the regulation of several bacterial operons. To obtain a deoxyribonucleic acid preparation enriched for the histidine (
his
) and for the isoleucine-valine (
ilv
) operons, we have isolated bacteriophages carrying the
his
and the
ilv
regions of the
Escherichia coli
chromosome. Transposition of the
his
operon to a site close to the
att80
region of the
E. coli
chromosome has been carried out selecting for integration of a temperature-sensitive F′
his
+
in the
tonB
locus. This transposed strain has been lysogenized with φ80i
λ
. Upon induction of the lysogen, His
+
transductants have been isolated, which, on further induction give rise to HFT (high frequency of transduction) lysates. Preliminary characterization of the transducing phage is reported. The
ilv
operon, carried on an F′ particle, has been fused to an episome carrying the
att80
region. The fused episome has been lysogenized with φ80i λt68. Upon induction of the lysogen, Ilv
+
transductants have been isolated which on further induction give rise to HFT lysates.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
34 articles.
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