Affiliation:
1. Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27607
Abstract
Studies were conducted on the oxidation and assimilation of various three-carbon compounds by a gram-positive rod isolated from soil and designated strain R-22. This organism can utilize propane, propionate, or
n
-propylamine as sole source of carbon and energy. Respiration rates, enzyme assays, and
14
CO
2
incorporation experiments suggest that propane is metabolized via methyl ketone formation; propionate and
n
-propylamine are metabolized via the methylmalonyl-succinate pathway. Isocitrate lyase activity was found in cells grown on acetate and was not present in cells grown on propionate or
n
-propylamine.
14
CO
2
was incorporated into pyruvate when propionate and
n
-propylamine were oxidized in the presence of NaAsO
2
, but insignificant radioactivity was found in pyruvate produced during the oxidation of propane and acetone. The
n
-propylamine dissimilatory mechanism was inducible in strain R-22, and amine dehydrogenase activity was detected in cells grown on
n
-propylamine. Radiorespirometer and
14
CO
2
incorporation studies with several propane-utilizing organisms indicate that the methylmalonyl-succinate pathway is the predominant one for the metabolism of propionate.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
35 articles.
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