Application of an objective biological assay of human interferons to clinical specimens and a survey of a normal population

Author:

Forti R L,Moldovan R A,Mitchell W M,Callicoat P,Schuffman S S,Davies H A,Smith D M

Abstract

A rapid, quantitative, and nonsubjective method of interferon assay is described, which can be readily applied to clinical specimens. Automated data acquisition and data reduction allowed a significant increase in volume per unit of time over existing methodologies. Plasma always yielded higher (usually 2:1) interferon values than did serum obtained simultaneously. Ranges of interferon levels in plasma in normal control populations are reported as well as ranges for clinical virology laboratory technicians and patients with terminal malignancies or collagen vascular diseases.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference6 articles.

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3. Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect;McManus N. H.;Appl. Environ. Microbiol.,1976

4. Mitchell W. M. and R. L. Forti. 1984. Progress in the monitoring of human interferon in body fluids and the phenotypic expression of human interferon activity p. 101-119. In M. Stefanini (ed.) Progress in clinical pathology vol. 9. Grune and Stratton New York.

5. Systemic Iupus erythematosus: presence in human serum of an unusual acid-labile leukocyte interferon;Preble 0.;Science,1982

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