Validation of a Gradient Diffusion Method (Etest) for Testing of Antimicrobial Susceptibility of Aerococcus urinae to Fluoroquinolones

Author:

Roy France Emilie1,Berteau Tammy1,Bestman-Smith Julie12,Grandjean Lapierre Simon345,Dufresne Simon Frédéric356,Domingo Marc-Christian7,Leduc Jean-Michel358ORCID

Affiliation:

1. Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université Laval, Quebec, Canada

2. Centre Hospitalier Universitaire de Québec-Université Laval, Quebec, Canada

3. Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université de Montréal, Montreal, Canada

4. Immunopathology Axis, Centre de Recherche du Centre Hospitalier de l’Université de Montréal, Montreal, Canada

5. Department of Laboratory Medicine, Centre Hospitalier de l'Université de Montréal, Montreal, Canada

6. Division of Infectious Diseases and Clinical Microbiology, Department of Medicine, Maisonneuve-Rosemont Hospital (CEMTL), Montreal, Canada

7. Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Ste-Anne-de-Bellevue, Canada

8. Centre de Recherche du Centre Intégré Universitaire de Santé et Services Sociaux du Nord-de-l’Île-de-Montréal, Hôpital du Sacré-Coeur de Montréal, Montreal, Canada

Abstract

Aerococcus urinae is a urinary pathogen with well-described resistance to fluoroquinolones. This study aimed to validate the gradient diffusion (GD) method (Etest) on cation-adjusted Mueller-Hinton agar with 5% sheep blood for testing the susceptibilities of Aerococcus urinae to the antimicrobial agents ciprofloxacin and levofloxacin and to compare the Etest to the broth microdilution (BMD) method from CLSI document M45-A3.

Funder

Biomerieux

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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