PCR engineering of viral quasispecies: a new method to preserve and manipulate genetic diversity of RNA virus populations-Reference-Cited by-同舟云学术

PCR engineering of viral quasispecies: a new method to preserve and manipulate genetic diversity of RNA virus populations

Published:1996-10 Issue:10 Volume:70 Page:7331-7334
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Chumakov K M¹

Affiliation:

1. Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20852, USA.

Abstract

A PCR-based method for the controlled manipulation of individual genomic sites of poliovirus with concomitant preservation of the sequence heterogeneity of the rest of the genome is proposed. The new approach can be used for the creation of stable DNA repositories of populations of extremely heterogenous RNA viruses and may have implications for live vaccine technology.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.70.10.7331-7334.1996

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