Cell-Free Synthesis of Encephalomyocarditis Virus-Reference-Cited by-同舟云学术

Cell-Free Synthesis of Encephalomyocarditis Virus

Published:2003-06 Issue:11 Volume:77 Page:6551-6555
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Svitkin Yuri V.¹,Sonenberg Nahum¹

Affiliation:

1. Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6

Abstract

ABSTRACT We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg 2+ and EMCV RNA concentrations. Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.77.11.6551-6555.2003

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