Ceftazidime and Aztreonam Resistance in Providencia stuartii : Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60-Reference-Cited by-同舟云学术

Ceftazidime and Aztreonam Resistance in Providencia stuartii : Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60

Published:1998-06 Issue:6 Volume:42 Page:1459-1462
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Franceschini Nicola¹,Perilli Mariagrazia¹,Segatore Bernardetta¹,Setacci Domenico¹,Amicosante Gianfranco¹,Mazzariol Annarita²,Cornaglia Giuseppe²

Affiliation:

1. Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell’Aquila, Loc. Coppito I-67010, L’Aquila,1 and

2. Istituto di Microbiologia, Università degli Studi di Verona, I-37134, Verona,2 Italy

Abstract

ABSTRACT A plasmid-encoded β-lactamase produced from a clinical strain of Providencia stuartii has been purified and characterized. The gene coding for the β-lactamase was cloned and sequenced. It appears to be a new natural TEM-derived enzyme, named TEM-60. Point mutations (Q39K, L51P, E104K, and R164S) are present with respect to the TEM-1 enzyme; the mutation L51P has never been previously reported, with the exception of the chromosomally encoded extended-spectrum β-lactamase PER-1. Kinetic parameters relative to penicillins, cephalosporins, and monobactams other than mechanism-based inactivators were related to the in vitro susceptibility phenotype.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.42.6.1459

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