Isolation of mycobacteria from undecontaminated specimens with selective 7H10 medium

Abstract

Media containing antimicrobial agents have been formulated for use as an adjunct to the standard media in an effort to reduce contamination and improve isolation of mycobacteria from clinical specimens. Selective 7H10 (S7H10) was developed for use in the isolation of mycobacteria from undecontaminated material. During a 33-month period, 10,782 clinical specimens were cultured in parallel on S7H10 without decontamination and on 7H11 after treatment with 2% sodium hydroxide-N-acetyl-L-cysteine. Results of this study show the overall contamination rate to be threefold lower on S7H10 than on 7H11 (304 versus 1,000). The number of specimens negative on NaOH-treated, 7H11-cultured specimens and contaminated on S7H10 was 282, whereas that negative on S7H10 but contaminated on NaOH-7H11 was 923. There were 6 positive cultures missed due to contamination on S7H10, compared with 61 on 7H11. Positive cultures on S7H10 outnumbered those on 7H11 by 106. This evaluation of S7H10 shows that it can be used with undecontaminated specimens in conjunction with standard methods and media for isolation of mycobacteria from clinical specimens.