Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis-Reference-Cited by-同舟云学术

Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis

Published:2020-09-22 Issue:10 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Blauenfeldt Thomas¹,Villar-Hernández Raquel²³⁴,García-García Esther²³⁴,Latorre Irene²³⁴,Holm Line Lindebo¹⁵,Muriel-Moreno Beatriz²³⁴,De Souza-Galvão Maria Luiza⁶,Millet Joan Pau⁷⁸,Sabriá Fina⁹,Sánchez-Montalva Adrián¹⁰,Ruiz-Manzano Juan³¹¹,Pilarte Jose⁶,Jiménez María A.⁶,Centeno Carmen¹¹,Torres Carmen⁹,Molina-Pinargote Israel⁷,González-Díaz Yoel D.⁷,Santiago Javier⁷,Cantos Adela⁶,Prat Cristina²³⁴,Andersen Peter¹,Domínguez Jose²³⁴^ORCID,Ruhwald Morten¹

Affiliation:

1. Department of Infectious Disease Immunology, Centre for Vaccine Research, Statens Serum Institut, Copenhagen, Denmark

2. Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d’Investigació Germans Trias i Pujol, Barcelona, Spain

3. CIBER Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Madrid, Spain

4. Universitat Autònoma de Barcelona, Barcelona, Spain

5. Department of Infectious Disease, Hvidovre Hospital, Hvidovre, Denmark

6. Unitat de Tuberculosi de Drassanes, Hospital Universitari Vall d’Hebron, Barcelona, Spain

7. Serveis Clínics, Unitat Clínica de Tractament Directament Observat de la Tuberculosi, Barcelona, Spain

8. CIBER de Epidemiología y Salud Pública, CIBEREESP, Instituto de Salud Carlos III, Madrid, Spain

9. Servei de Pneumologia, Hospital Sant Joan Despí Moises Broggi, Sant Joan Despi, Barcelona, Spain

10. Servei de Malalties Infeccioses, Hospital Universitari de la Vall d’Hebron, Barcelona, Spain

11. Servei de Pneumologia, Hospital Universitari Germans Trias i Pujol, Barcelona, Spain

Abstract

Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 100-fold-higher expression level than IFN-γ, allowing for different analysis platforms, including molecular detection. The PCR technique is already an integrated tool in most TB laboratories and, thus, an obvious platform to turn to.

Funder

The Research Council of Norway

European Comission H2020 Programme

MEC | Instituto de Salud Carlos III

Ministry of Health | Statens Serum Institut

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00848-20

Reference33 articles.