How To Optimally Combine Genotypic and Phenotypic Drug Susceptibility Testing Methods for Pyrazinamide-Reference-Cited by-同舟云学术

How To Optimally Combine Genotypic and Phenotypic Drug Susceptibility Testing Methods for Pyrazinamide

Published:2020-08-20 Issue:9 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Köser Claudio U.¹^ORCID,Cirillo Daniela M.²^ORCID,Miotto Paolo²^ORCID

Affiliation:

1. Department of Genetics, University of Cambridge, Cambridge, United Kingdom

2. Emerging Bacterial Pathogens Unit, IRCCS Ospedale San Raffaele, Milan, Italy

Abstract

False-susceptible phenotypic drug-susceptibility testing (DST) results for pyrazinamide due to mutations with MICs close to the critical concentration (CC) confound the classification of pncA resistance mutations, leading to an underestimate of the specificity of genotypic DST. This could be minimized by basing treatment decisions on well-understood mutations and by adopting an area of technical uncertainty for phenotypic DST rather than only testing the CC, as is current practice for the Mycobacterium tuberculosis complex.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01003-20

Reference35 articles.

1. The Bewildering Antitubercular Action of Pyrazinamide

2. World Health Organization. 2018. Technical manual for drug susceptibility testing of medicines used in the treatment of tuberculosis. World Health Organization, Geneva, Switzerland. http://apps.who.int/iris/bitstream/handle/10665/275469/9789241514842-eng.pdf.

3. Using Reduced Inoculum Densities of Mycobacterium tuberculosis in MGIT Pyrazinamide Susceptibility Testing to Prevent False-Resistant Results and Improve Accuracy: A Multicenter Evaluation

4. Drug-Resistant Tuberculosis, Lebanon, 2016 – 2017

5. Reply to Anthony et al., “Protecting Pyrazinamide, a Priority for Improving Outcomes in Multidrug-Resistant Tuberculosis Treatment”

Cited by 20 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Lineage classification and antitubercular drug resistance surveillance of Mycobacterium tuberculosis by whole-genome sequencing in Southern India;Microbiology Spectrum;2023-10-17

2. Resistance patterns and transmission of mono- and polyresistant TB: clinical impact of WGS;JAC-Antimicrobial Resistance;2023-09-04

3. Drug resistance prediction for Mycobacterium tuberculosis with reference graphs;Microbial Genomics;2023-08-08

4. A Bayesian approach to estimate the probability of resistance to bedaquiline in the presence of a genomic variant;PLOS ONE;2023-06-14

5. Drug resistance prediction forMycobacterium tuberculosiswith reference graphs;2023-05-04