Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA-Reference-Cited by-同舟云学术

Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA

Published:1989-07 Issue:7 Volume:55 Page:1818-1822
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Weller Roland¹,Ward David M.¹

Affiliation:

1. Department of Microbiology, Montana State University, Bozeman, Montana 59717

Abstract

Cloning of cDNA obtained from 16S rRNA (16S rcDNA) selectively retrieves species-specific sequence information useful for analyzing the composition and structure of natural microbial communities. With this technique we obtained recombinant 16S rcDNA libraries from Escherichia coli and from a model hot-spring cyanobacterial-mat community. The recombinant plasmids contained exclusively 16S rRNA-derived inserts. This selective approach is independent of biasing culture techniques and eliminates the laborious screening required to locate 16S rRNA gene-bearing recombinants in genomic DNA libraries obtained from natural communities.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.55.7.1818-1822.1989

Reference25 articles.

1. Atlas R. M. 1984. Use of microbial diversity measurements to assess environmental stress p. 540-545. In M. J. Klug and C. A. Reddy (ed.) Current perspectives in microbial ecology. American Society for Microbiology Washington D.C.

2. Creating a ribonuclease-free environment;Blumberg D. D.;Methods Enzymol.,1987

3. The study of microorganisms in situ: progress and problems;Brock T. D.;Symp. Soc. Gen. Microbiol.,1987

4. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli;Brosius J.;Proc. Natl. Acad. Sci. USA,1978

5. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells;DeLong E. F.;Science,1989

Cited by 98 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Microbial community composition unaffected by mycorrhizal plant removal in sub-arctic tundra;Fungal Ecology;2024-06

2. Ribovore: ribosomal RNA sequence analysis for GenBank submissions and database curation;BMC Bioinformatics;2021-08-12

3. CaptureSeq: Hybridization-Based Enrichment of cpn60 Gene Fragments Reveals the Community Structures of Synthetic and Natural Microbial Ecosystems;Microorganisms;2021-04-13

4. Ribovore: ribosomal RNA sequence analysis for GenBank submissions and database curation;2021-02-12

5. Issues and Perspectives on Environmental Microbial Dynamics and Groundwater Flow System Research;Journal of Groundwater Hydrology;2020-08-31