Description of a Nonlethal Herpes Simplex Virus Type 1 Glycoprotein D Deletion Mutant Affecting a Site Frequently Used for PCR-Reference-Cited by-同舟云学术

Description of a Nonlethal Herpes Simplex Virus Type 1 Glycoprotein D Deletion Mutant Affecting a Site Frequently Used for PCR

Published:2000-03 Issue:2 Volume:7 Page:322-324
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Coyle P. V.¹,Jain S.¹,Wyatt D.¹,McCaughey C.¹,O'Neill H. J.¹

Affiliation:

1. Regional Virus Laboratory, Royal Victoria Hospital, Belfast, United Kingdom

Abstract

ABSTRACT We report a herpes simplex virus type 1 mutant which failed to amplify with a commonly used glycoprotein D primer set. The virus contained a nine-base deletion in the gene's 5′ nontranslated region. The altered amplicon was clearly distinguishable on a 4% high-resolution agarose gel.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.7.2.322-324.2000

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