Identification of Potentially Diagnostic Leishmania braziliensis Antigens in Human Cutaneous Leishmaniasis by Immunoblot Analysis-Reference-Cited by-同舟云学术

Identification of Potentially Diagnostic Leishmania braziliensis Antigens in Human Cutaneous Leishmaniasis by Immunoblot Analysis

Published:2000-03 Issue:2 Volume:7 Page:318-321
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Brito Maria Edileuza F.¹,Mendonça Mitzi G.²,Gomes Yara M.¹,Jardim Márcio L.²,Abath Frederico G. C.¹

Affiliation:

1. Departmento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz,1 and

2. Departmento de Dermatologia, Universidade Federal de Pernambuco,2 Cidade Universitária, 50670-420 Recife, Brazil

Abstract

ABSTRACT The antibody response in patients with American cutaneous leishmaniasis was analyzed by immunoblotting with soluble and insoluble antigens of Leishmania braziliensis . The recognition of the 27- and/or 30-kDa soluble antigens was considered relevant for the diagnosis of cutaneous leishmaniasis. Immunoblotting was found to be significantly more sensitive and specific than indirect immunofluorescence and enzyme-linked immunosorbent assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.7.2.318-321.2000

Reference22 articles.

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