Use of Green Fluorescent Protein To Detect Expression of an Endopolygalacturonase Gene of Colletotrichum lindemuthianum during Bean Infection-Reference-Cited by-同舟云学术

Use of Green Fluorescent Protein To Detect Expression of an Endopolygalacturonase Gene of Colletotrichum lindemuthianum during Bean Infection

Published:1999-04 Issue:4 Volume:65 Page:1769-1771
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Dumas Bernard¹,Centis Sylvie¹,Sarrazin Nathalie¹,Esquerré-Tugayé Marie-Thérèse¹

Affiliation:

1. UMR 5546 CNRS-UPS, “Signaux et Messages Cellulaires chez les Végétaux,” Pôle de Biotechnologie Végétale, Auzeville 31326, Castanet-Tolosan, France

Abstract

ABSTRACT The 5′ noncoding region of clpg2 , an endopolygalacturonase gene of the bean pathogen Colletotrichum lindemuthianum , was fused to the coding sequence of a gene encoding a green fluorescent protein (GFP), and the construct was introduced into the fungal genome. Detection of GFP accumulation by fluorescence microscopy examination revealed that clpg2 was expressed at the early stages of germination of the conidia and during appressorium formation both in vitro and on the host plant.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.4.1769-1771.1999

Reference16 articles.

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4. Endopolygalacturonase genes from Colletotrichum lindemuthianum: cloning of CLPG2 and comparison of its expression to that of CLPG1 during saprophytic and parasitic growth of the fungus.;Centis S.;Mol. Plant-Microbe Interact.,1997

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