Determinants of Entry Exclusion within Eex and TraG Are Cytoplasmic-Reference-Cited by-同舟云学术

Determinants of Entry Exclusion within Eex and TraG Are Cytoplasmic

Published:2007-09 Issue:17 Volume:189 Page:6469-6473
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Marrero Joeli¹,Waldor Matthew K.²

Affiliation:

1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine

2. Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts

Abstract

ABSTRACT We report here functional and topological analyses of TraG and Eex, the donor and recipient cell inner membrane proteins that mediate entry exclusion in the SXT/R391 family of integrative conjugative elements. We found that the exclusion-determining regions of the Eex variants EexS (SXT) and EexR (R391) are located in distinct yet overlapping regions of the proteins. Unexpectedly, the carboxyl-terminal regions of TraG and Eex, which contain the residues essential for exclusion activity and specificity, were found to localize in the cell cytoplasm. These observations suggest that complex topological rearrangements of conjugative proteins must occur during mating to enable these domains to interact.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00522-07

Reference27 articles.

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