Affiliation:
1. Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907
Abstract
ABSTRACT
Baylisascaris procyonis
and
Toxocara
species are two important causes of larva migrans in humans. Larva migrans caused by
Toxocara
spp. is well known and is diagnosed serologically by enzyme immunoassay. Over a dozen cases of larva migrans and associated eosinophilic encephalitis caused by
B. procyonis
have also been reported, and at least a dozen additional cases are known. An enzyme-linked immunosorbent assay (ELISA) using the excretory-secretory (ES) antigen of
B. procyonis
larvae is currently being used in our laboratory as an aid in the diagnosis of this infection in humans. Clinically affected individuals show very high reactivity (measured as the optical density) on this ELISA; however, a one-way cross-reactivity with
Toxocara
spp. has been observed. As an approach to differentiate these two infections based on serology, we performed Western blots, wherein the
B. procyonis
ES antigen was reacted with serum samples from individuals known to be positive for either
Toxocara
spp. or
B. procyonis
larva migrans. Western blot results showed that
B. procyonis
antigens of between 30 and 45 kDa were specifically identified only by the sera from individuals with
Baylisascaris
larva migrans, thus allowing for differentiation between the two infections. This included human patient serum samples submitted for serologic testing, as well as sera from rabbits experimentally infected with
B. procyonis
. When used in conjunction with the ELISA, Western blotting could be an efficient tool for diagnosis of this infection in humans.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
Reference29 articles.
1. Beaver, P. C. 1969. The nature of visceral larva migrans. J. Parasitol.55:3-12.
2. Boyce, W. M., D. J. Asai, J. K. Wilder, and K. R. Kazacos. 1989. Physicochemical characterization and monoclonal and polyclonal antibody recognition of Baylisascaris procyonis larval excretory-secretory antigens. J. Parasitol.75:540-548.
3. Boyce, W. M., B. A. Branstetter, and K. R. Kazacos. 1988. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis, and Ascaris suum by Western blotting and enzyme immunoassay. Int. J. Parasitol.18:109-113.
4. Boyce, W. M., B. A. Branstetter, and K. R. Kazacos. 1988. In vitro culture of Baylisascaris procyonis and initial analysis of larval excretory-secretory antigens. Proc. Helminthol. Soc. Wash.55:15-18.
5. Centers for Disease Control and Prevention. 2002. Raccoon roundworm encephalitis-Chicago, Illinois, and Los Angeles, California, 2000. MMWR Morb. Mortal. Weekly Rep.50:1153-1155.
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