Establishment of a Highly Sensitive Assay for Detection of Hepatitis E Virus-Specific Immunoglobulins-Reference-Cited by-同舟云学术

Establishment of a Highly Sensitive Assay for Detection of Hepatitis E Virus-Specific Immunoglobulins

Published:2020-01-28 Issue:2 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bohm Katrin¹,Strömpl Julia¹,Krumbholz Andi²,Zell Roland³,Krause Gérard¹⁴⁵^ORCID,Sievers Claudia¹

Affiliation:

1. Department of Epidemiology, Helmholtz Centre for Infectious Research, Brunswick, Germany

2. Institute of Infection Medicine, University of Kiel, University Hospital Schleswig Holstein, Kiel, Germany

3. Division of Experimental Virology, Institute of Medical Microbiology, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany

4. Institute for Infectious Disease Epidemiology, TWINCORE, Hanover, Germany

5. Translational Infrastructure Epidemiology, German Centre for Infection Research (DZIF), Brunswick, Germany

Abstract

Hepatitis E, a liver disease caused by infection with the hepatitis E virus (HEV), is a worldwide emerging disease. The diagnosis is based on the detection of viral RNA and of HEV-specific immunoglobulins (Ig). For the latter, various assays are commercially available but still lack harmonization. In this study, a Luminex-based multiplex serological assay was established that measures the presence of total IgG, IgA, and IgM antibodies, targeting a short peptide derived from the viral E2 protein.

Funder

Deutsches Zentrum für Infektionsforschung

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01029-19

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