Affiliation:
1. Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
Abstract
ABSTRACT
Appropriate responses of organisms to heat stress are essential for their survival. In eukaryotes, adaptation to high temperatures is mediated by heat shock transcription factors (HSFs). HSFs regulate the expression of heat shock proteins, which function as molecular chaperones assisting in protein folding and stability. In many model organisms a great deal is known about the products of
hsf
genes. An important exception is the filamentous fungus and model eukaryote
Neurospora crassa
. Here we show that two
Neurospora crassa
genes whose protein products share similarity to known HSFs play different biological roles. We report that
heat shock factor 1
(
hsf1
) is an essential gene and that
hsf2
is required for asexual development. Conidiation may be blocked in the
hsf2
knockout (
hsf2
KO
) strain because HSF2 is an integral element of the conidiation pathway or because it affects the availability of protein chaperones. We report that genes expressed during conidiation, for example
fluffy
,
conidiation-10
, and
repressor of conidiation-1
show wild-type levels of expression in a
hsf2
KO
strain. However, consistent with the lack of macroconidium development, levels of
eas
are much reduced. Cultures of the
hsf2
KO
strain along with two other aconidial strains, the
fluffy
and
aconidial-2
strains, took longer than the wild type to recover from heat shock. Altered expression profiles of
hsp90
and a putative
hsp90
-associated protein in the
hsf2
KO
strain after exposure to heat shock may in part account for its reduced ability to cope with heat stress.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
15 articles.
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