New Culture Medium Containing Ionic Concentrations of Nutrients Similar to Concentrations Found in the Soil Solution-Reference-Cited by-同舟云学术

New Culture Medium Containing Ionic Concentrations of Nutrients Similar to Concentrations Found in the Soil Solution

Published:1991-12 Issue:12 Volume:57 Page:3674-3676
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Angle J. Scott¹,McGrath Stephen P.¹,Chaney Rufus L.¹

Affiliation:

1. Department of Agronomy, University of Maryland, College Park, Maryland 20742; Soils and Agronomy Department, AFRC Institute of Arable, Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ, United Kingdom2; and Environmental Chemistry Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 207053

Abstract

A new growth medium which closely approximates the composition of the soil solution is presented. This soil solution equivalent (SSE) medium contains the following components (millimolar): NO 3 , 2.5; NH 4 , 2.5; HPO 4 , 0.005; Na, 2.5; Ca, 4.0; Mg, 2.0; K, 0.503; Cl, 4.0; SO 4 , 5.0; ethylenediamine-di( o -hydroxyphenylacetic acid), 0.02; and MES [2-( N -morpholino)ethanesulfonic acid] (to maintain the pH at 6.0), 10, plus 0.1% arabinose. The advantages of the SSE medium are discussed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.57.12.3674-3676.1991

Reference17 articles.

1. Cadmium resistance screening in nitrilotriacetate-buffered minimal media;Angle J. S.;Appl. Environ. Microbiol.,1989

2. la.Chaney R. L. Personal communication.

3. Plants can utilize iron from Fe-N,N,di-(2- hydroxybenzoil)-ethylenediamine-N-diacetic acid, a ferric chelate with 106 greater formation constant than FeEDDHA;Chaney R. L.;J. Plant Nutr.,1988

4. Transmissible resistance to penicillin G, neomycin, and chloramphenicol in Rhizobium japonicum;Cole M. A.;Antimicrob. Agents Chemother.,1973

5. Soil-plant relationships in ion uptake;Fried M.;Plant Physiol.,1961

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