Phage abortive infection mechanism from Lactococcus lactis subsp. lactis, expression of which is mediated by an Iso-ISS1 element

Author:

Cluzel P J1,Chopin A1,Ehrlich S D1,Chopin M C1

Affiliation:

1. Laboratoire de Génétique Microbienne, Institut de Biotechnologie, I.N.R.A., Jouy-en-Josas, France.

Abstract

A 5-kb DNA fragment conferring a phage abortive infection phenotype (Abi+) has been cloned from Lactococcus lactis subsp. lactis IL416. The Abi+ determinant was subcloned on a 2-kb fragment which carried an Iso-ISS1 element and an open reading frame of 753 bp designated ORFX. Deletion within ORFX entailed the loss of the Abi+ phenotype, establishing that ORFX is the structural abi-416 gene. The expression of abi-416 was shown to be mediated by the Iso-ISS1 element, which contains a sequence fitting the consensus sequence for gram-positive promoters.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference45 articles.

1. Genetic and physical characterization of recombinant plasmids associated with cell aggregation and high-frequency conjugal transfer in Streptococcus lactis ML3;Anderson D. G.;J. Bacteriol.,1984

2. IS431, a staphylococcal insertion sequence-like element related to IS26 from Proteus vulgaris;Barberis-Maino L.;Gene,1987

3. Two plasmid-determined restriction and modification systems in Streptococcus lactis;Chopin A.;Plasmid,1984

4. Daly C. and G. Fitzgerald. 1987. Mechanisms of bacteriophage insensitivity in the lactic streptococci p. 259-268. In J. Ferretti and R. Curtiss III (ed.) Streptococcal genetics. American Society for Microbiology Washington D.C.

5. Nucleotide sequence and characterization of a new insertion element, IS240, from Bacillus thuringiensis israelensis;Delecluse A.;Plasmid,1989

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3