Expression of H C Subunits from Clostridium botulinum Types C and D and Their Evaluation as Candidate Vaccine Antigens in Mice-Reference-Cited by-同舟云学术

Expression of H C Subunits from Clostridium botulinum Types C and D and Their Evaluation as Candidate Vaccine Antigens in Mice

Published:2003-05 Issue:5 Volume:71 Page:2941-2944
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Woodward L. A.¹²,Arimitsu H.²,Hirst R.¹,Oguma K.²

Affiliation:

1. Department of Microbiology and Immunology, James Cook University, Townsville 4811, Australia

2. Department of Bacteriology, Graduate School of Medicine and Dentistry, Okayama University, Okayama 700-8558, Japan

Abstract

ABSTRACT Two proteins representing the heavy-chain subunits of botulinum neurotoxin types C and D were expressed in Escherichia coli , and their vaccine potential was evaluated. Mice were vaccinated with doses ranging from 0.5 to 10 μg and were challenged with 10 to 10 5 50% lethal doses of toxin. For the type C subunit protein, C50, two doses of 2 μg were required for full protection, while, for type D subunit protein, D50, two 1-μg doses were required. A bivalent vaccine consisting of a mixture of these two proteins also provided protection against both botulinum neurotoxin type C and type D challenge. Antibody levels in serum were determined by both enzyme-linked immunosorbent assays and serum neutralization assays

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.71.5.2941-2944.2003

Reference15 articles.

1. Interaction of 125I-labeled botulinum neurotoxins with nerve terminals. I. Ultrastructural autoradiographic localization and quantitation of distinct membrane acceptors for types A and B on motor nerves.

2. Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli

3. Craven, C. 1964. Control of a botulinum-like disease in north Queensland. Aust. Vet. J.40:127-130.

4. DasGupta B. R. 1989. The structure of botulinum neurotoxins p. 53-67. In L. L. Simpson (ed.) Botulinum neurotoxin and tetanus toxin. Academic Press New York N.Y.

5. DasGupta, B. R., and H. Sugiyama. 1972. A common subunit structure in Clostridium botulinum type A, B and E toxins. Biochem. Biophys. Res. Commun.48:108-112.

Cited by 34 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Cloning and expression of immunogenic Clostridium botulinum C2I mutant proteins designed from their evolutionary imprints;Comparative Immunology, Microbiology and Infectious Diseases;2019-08

2. Immunoproteomics Approach for Screening of Vaccine Candidates against Intestinal Botulism;Protein & Peptide Letters;2017-10-24

3. Production of recombinant botulism antigens: A review of expression systems;Anaerobe;2014-08

4. High level expression, purification and immunogenicity analysis of a protective recombinant protein against botulinum neurotoxin type E;World Journal of Microbiology and Biotechnology;2014-01-29

5. Production and Evaluation of a Recombinant Chimeric Vaccine against Clostridium botulinum Neurotoxin Types C and D;PLoS ONE;2013-07-31