Myb-Related Schizosaccharomyces pombe cdc5p Is Structurally and Functionally Conserved in Eukaryotes

Author:

Ohi Ryoma1,Feoktistova Anna2,McCann Stacey3,Valentine Virginia4,Look A. Thomas4,Lipsick Joseph S.3,Gould Kathleen L.21

Affiliation:

1. Department of Cell Biology, 1 School of Medicine, Vanderbilt University, Nashville, Tennessee 37232;

2. Howard Hughes Medical Institute 2 and

3. Department of Pathology and Program in Molecular and Genetic Medicine, School of Medicine, Stanford University, Stanford, California 94305 3 ; and

4. Department of Experimental Oncology, St. Jude Children’s Research Hospital, Memphis, Tennessee 381054

Abstract

ABSTRACT Schizosaccharomyces pombe cdc5p is a Myb-related protein that is essential for G 2 /M progression. To explore the structural and functional conservation of Cdc5 throughout evolution, we isolated Cdc5-related genes and cDNAs from Saccharomyces cerevisiae , Caenorhabditis elegans , Drosophila melanogaster , and Homo sapiens . Supporting the notion that these Cdc5 gene family members are functionally homologous to S. pombe cdc5 + , human and fly Cdc5 cDNAs are capable of complementing the temperature-sensitive lethality of the S. pombe cdc5-120 mutant. Furthermore, S. cerevisiae CEF1 ( S. cerevisiae homolog of cdc5 + ), like S. pombe cdc5 + , is essential during G 2 /M. The location of the cdc5-120 mutation, as well as mutational analyses of Cef1p, indicate that the Myb repeats of cdc5p and Cef1p are important for their function in vivo. However, we found that unlike in c-Myb, single residue substitutions of glycines for hydrophobic residues within the Myb repeats of Cef1p, which are essential for maintaining structure of the Myb domain, did not impair Cef1p function in vivo. Rather, multiple W-to-G substitutions were required to inactivate Cef1p, and many of the substitution mutants were found to confer temperature sensitivity. Although it is possible that Cef1p acts as a transcriptional activator, we have demonstrated that Cef1p is not involved in transcriptional activation of a class of G 2 /M-regulated genes typified by SWI5 . Collectively, these results suggest that Cdc5 family members participate in a novel pathway to regulate G 2 /M progression.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Reference69 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3