Improved Susceptibility Disk Assay Method Employing an Agar Overlay Technique-Reference-Cited by-同舟云学术

Improved Susceptibility Disk Assay Method Employing an Agar Overlay Technique

Published:1978-11 Issue:5 Volume:14 Page:761-764
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Anderson Blondell¹

Affiliation:

1. Antibiotic Residue Branch, National Center for Antibiotics Analysis, Food and Drug Administration, Washington, D.C. 20204

Abstract

Susceptibility disks impregnated with 35 different antibiotics were assayed by the currentCode of Federal Regulationsmethod and by a new method employing an agar overlay. The new method, a simplified analytical procedure, exhibited improvement in zone definition and an increase in dose response. Assays performed by the new method were accurate and precise. The agar overlay technique appears to be superior to the currentCode of Federal Regulationsmethod.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.14.5.761

Reference4 articles.

1. An improved single-disk method for testing antibiotic susceptibility of rapidly-growing pathogens;Barry A. L.;Am. J. Clin. Pathol.,1970

2. Lorian V. 1966. Antibiotics and chemotherapeutic agents in clinical and laboratory practice. Charles C Thomas Publisher Springfield Ill.

3. Effects of paper on performance of antibiotic-impregnated disks;Rippere R. A.;J. Pharm. Sci.,1978

4. United States Government Printing Office. 1975. Code of Federal Regulations. Title 21 Section 460. United States Government Printing Office Washington D.C.

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1. Accurate assessment of antibiotic susceptibility and screening resistant strains of a bacterial population by linear gradient plate;Science China Life Sciences;2011-10

2. Application of the disk method to cultured plant cells. I. Inhibition zones;Plant Cell, Tissue and Organ Culture;1984

3. A new rapid agar cup assay of β-lactam antibiotics in the presence of a chemical reagent;Analytical Biochemistry;1980-10