Characterization of Phospholipase Activity of the Pseudomonas aeruginosa Type III Cytotoxin, ExoU-Reference-Cited by-同舟云学术

Characterization of Phospholipase Activity of the Pseudomonas aeruginosa Type III Cytotoxin, ExoU

Published:2005-02 Issue:3 Volume:187 Page:1192-1195
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Sato Hiromi¹,Feix Jimmy B.²,Hillard Cecilia J.³,Frank Dara W.¹

Affiliation:

1. Department of Microbiology and Molecular Genetics

2. Department of Biophysics

3. Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin

Abstract

ABSTRACT Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.187.3.1192-1195.2005

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