Kinetics of the Immune Response Associated with Tularemia: Comparison of an Enzyme-Linked Immunosorbent Assay, a Tube Agglutination Test, and a Novel Whole-Blood Lymphocyte Stimulation Test-Reference-Cited by-同舟云学术

Kinetics of the Immune Response Associated with Tularemia: Comparison of an Enzyme-Linked Immunosorbent Assay, a Tube Agglutination Test, and a Novel Whole-Blood Lymphocyte Stimulation Test

Published:2008-08 Issue:8 Volume:15 Page:1238-1243
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Eliasson Henrik¹²³⁴⁵,Olcén Per¹²³⁴⁵,Sjöstedt Anders¹²³⁴⁵,Jurstrand Margareta¹²³⁴⁵,Bäck Erik¹²³⁴⁵,Andersson Sören¹²³⁴⁵

Affiliation:

1. Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden

2. Department of Clinical Microbiology and Immunology, Örebro University Hospital, Örebro, Sweden

3. Department of Clinical Medicine, Örebro University, Örebro, Sweden

4. Department of Clinical Microbiology, Umeå University Hospital, Umeå, Sweden

5. Clinical Research Centre, Örebro University Hospital, Örebro, Sweden

Abstract

ABSTRACT We have developed and evaluated a novel and simplified whole-blood lymphocyte stimulation assay that focuses on the measurement of gamma interferon after 24 h of stimulation with whole-cell tularemia antigen and a tularemia enzyme-linked immunosorbent assay (ELISA) based on highly purified lipopolysaccharide antigen. Comparison of the kinetics of the two assays and those of the traditional tube agglutination test shows that the cellular immune response can be detected earlier by the lymphocyte stimulation assay. This test already shows a high proportion of positive results during the first week after the onset of the disease, may be applicable in everyday laboratory practice, and has the potential of changing routine diagnostics for tularemia. The new ELISA has a high sensitivity and becomes positive to a high degree during the second week of disease.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00434-07

Reference31 articles.

1. Comparative analysis of antibodies to Francisella tularensis antigens during the acute phase of tularemia and eight years later

2. Buchanan, T. M., G. F. Brooks, and P. S. Brachman. 1971. The tularemia skin test. 325 skin tests in 210 persons: serologic correlation and review of the literature. Ann. Intern. Med.74:336-343.

3. Enzyme-Linked Immunosorbent Assay for Immunological Diagnosis of Human Tularemia

4. Eliasson, H., T. Broman, M. Forsman, and E. Bäck. 2006. Tularemia: current epidemiology and disease management. Infect. Dis. Clin. N. Am.20:289-311, ix.

5. Eliasson, H., and E. Bäck. 2007. Tularaemia in an emergent area in Sweden: an analysis of 234 cases in five years. Scand. J. Infect. Dis.39:880-889.

Cited by 14 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Thoracic manifestations of tularaemia: a case series;Infection;2024-03-08

2. Tulareminin Serolojik Tanısı İçin In House Enzyme- Linked Immunosorbent Assay (ELISA) Prototipi ve Mikroaglütinasyon Test (MAT) Antijeninin Geliştirilmesi;Journal of Research in Veterinary Medicine;2021-05-04

3. Evaluation of In-House and Commercial Serological Tests for Diagnosis of Human Tularemia;Journal of Clinical Microbiology;2018-01

4. Cat-bite-induced Francisella tularensis infection with a false-positive serological reaction for Bartonella quintana;JMM Case Reports;2017-02-28

5. Francisella;Manual of Clinical Microbiology;2015-05-26