Rapid Extraction Method with Pronase B for Grouping Beta-Hemolytic Streptococci-Reference-Cited by-同舟云学术

Rapid Extraction Method with Pronase B for Grouping Beta-Hemolytic Streptococci

Published:1972-02 Issue:2 Volume:23 Page:285-288
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Ederer Grace Mary¹²³,Herrmann Mary M.¹²³,Bruce Robert¹²³,Matsen John M.¹²³,Chapman S. Stephen¹²³

Affiliation:

1. Department of Laboratory Medicine, University of Minnesota, Minneapolis, Minnesota, 55455

2. Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, 55455

3. Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, 55455

Abstract

The enzyme, Pronase B, was used to extract the C carbohydrates of betahemolytic streptococci for serological grouping. Reference strains of streptococci, groups A through N, were accurately grouped by using this extraction method. Of the beta-hemolytic streptococci isolated from patients' cultures, 1,197 were classified as either group A or not group A by this method. There was 100% correlation with the reference Lancefield method. In contrast, false reactions occurred with the presumptive bacitracin disc method in 4.1% of the group A and 12.7% of the non-group A cultures. The Pronase method proved simple, accurate, and readily adaptable to the clinical laboratory routine.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.23.2.285-288.1972

Reference13 articles.

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