A CRISPR/Cas9-Mediated, Homology-Independent Tool Developed for Targeted Genome Integration in Yarrowia lipolytica-Reference-Cited by-同舟云学术

A CRISPR/Cas9-Mediated, Homology-Independent Tool Developed for Targeted Genome Integration in Yarrowia lipolytica

Published:2021-02-26 Issue:6 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cui Zhiyong¹,Zheng Huihui¹,Zhang Jinhong¹,Jiang Zhennan¹,Zhu Ziwei¹,Liu Xiaoqin¹,Qi Qingsheng¹²^ORCID,Hou Jin¹

Affiliation:

1. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People’s Republic of China

2. CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, People’s Republic of China

Abstract

This study describes the development and optimization of a homology-independent targeted genome integration tool mediated by CRISPR/Cas9 in Yarrowia lipolytica . This tool does not require the construction of homologous templates and can be used to rapidly verify genetic elements and to iteratively integrate multiple-gene pathways in Y. lipolytica . This tool may serve as a potential supplement to current HR-dependent genome-editing methods for eukaryotes.

Funder

National Defense Science and Technology Innovation Zone Foundation of China

China Postdoctoral Science Foundation

National Natural Science Foundation of China

MOST | National Key Research and Development Program of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02666-20

Reference50 articles.