In Vivo Modulation of a DnaJ Homolog, CbpA, by CbpM-Reference-Cited by-同舟云学术

In Vivo Modulation of a DnaJ Homolog, CbpA, by CbpM

Published:2007-05 Issue:9 Volume:189 Page:3635-3638
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Chenoweth Matthew R.¹,Trun Nancy²,Wickner Sue¹

Affiliation:

1. Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892

2. Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282

Abstract

ABSTRACT CbpA, an Escherichia coli DnaJ homolog, can function as a cochaperone for the DnaK/Hsp70 chaperone system, and its in vitro activity can be modulated by CbpM. We discovered that CbpM specifically inhibits the in vivo activity of CbpA, preventing it from functioning in cell growth and division. Furthermore, we have shown that CbpM interacts with CbpA in vivo during stationary phase, suggesting that the inhibition of activity is a result of the interaction. These results reveal that the activity of the E. coli DnaK system can be regulated in vivo by a specific inhibitor.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01757-06

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