Author:
Olsen R H,DeBusscher G,McCombie W R
Abstract
A host-vector system for Pseudomonas aeruginosa PAO was developed. Scattered regions of the strain PAO chromosome were cloned by direct selection for complementation of auxotrophs or from a DNA gene bank which contains over 1,000 independently isolated chromosome-vector recombinant plasmids. The use of partially digested chromosomal DNA facilitated the selection of a variety of strain PAO chromosomal markers. The progenitor of the vector was a small, multicopy plasmid, pRO1600, found in a PAO strain which had acquired RP1 in a mating experiment. The bacterial host range that could be determined by transformation of vectors produced from pRO1600 resembles that for plasmid RP1. Two derivative plasmids were formed: pRO1613, for cloning DNA cleaved with restriction endonuclease PstI, and pRO1614, which was formed by deleting part of pRO1613 and fusion with plasmid pBR322. Plasmid pRO1614 utilizes known cloning sites within the tetracycline resistance region of pBR322.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference23 articles.
1. New vector plasmids for gene cloning;Bagd M.;Pseudomonas. Dev. Genet.,1979
2. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system;Bolivar R.;Gene (Amst.),1977
3. Relationship of group Pl plasmids revealed by heteroduplex experiments: RP1, RP4, R68, and RK2 are identical;Burkhardt H.;J. Gen. Microbiol.,1979
4. Carbon J. L. Clarke C. 41en and B. Ratzlcn. 1977. The construction and use of hybrid plasmid gene banks in Escherichia coli. p. 355-378. In R. Beers and E. Bassett (ed.) Recombinant molecules: impact on science and society. Raven Press New York.
5. Isolation and properties of recombination-deficient mutants of Pseudomonas aeruginosa;Chandler P.;Mutat. Res.,1974
Cited by
217 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献