Affiliation:
1. Observatoire Burkholderia cepacia, Laboratoire de Bactériologie-Hygiène, Hôpital Purpan, Toulouse, France
2. Laboratoire de Bactériologie, Hôpital Jean Minjoz, Besançon, France
3. Service de Pneumologie, Hôpital Foch, Suresnes, France
4. Laboratoire de Biologie, Centre Hospitalier, Cahors, France
Abstract
ABSTRACT
Burkholderia gladioli
, primarily known as a plant pathogen, is involved in human infections, especially in patients with cystic fibrosis (CF). In the present study, the first respiratory isolates recovered from 14 French patients with CF and 4 French patients without CF, identified by 16S rRNA gene analysis, were tested for growth on
B. cepacia
selective media, for identification by commercial systems, and for their antimicrobial susceptibilities, and were compared by pulsed-field gel electrophoresis (PFGE). Patients' data were collected. All 18 isolates grew on oxidation-fermentation-polymyxin B-bacitracin-lactose medium and
Pseudomonas cepacia
agar, but only 13 grew on
Burkholderia cepacia
selective agar. API 20NE strips did not differentiate
B. gladioli
from
B. cepacia
, whereas Vitek 2 GN cards correctly identified 15 isolates. All isolates were susceptible to piperacillin, imipenem, aminoglycosides, and ciprofloxacin and were far less resistant to ticarcillin than
B. cepacia
complex organisms. Fifteen PFGE types were observed among the 18 isolates, but shared types were not identified among epidemiologically related patients. The microbiological follow-up of CF patients showed that colonization was persistent in 3 of 13 documented cases;
B. gladioli
was isolated from posttransplantation cultures of blood from 1 patient. Among the patients without CF,
B. gladioli
was associated with intubation (three cases) or bronchiectasis (one case). In summary, the inclusion of
B. gladioli
in the databases of commercial identification systems should improve the diagnostic capabilities of those systems. In CF patients, this organism is more frequently involved in transient infections than in chronic infections, but it may be responsible for complications posttransplantation; patient-to-patient transmission has not been demonstrated to date. Lastly,
B. gladioli
appears to be naturally susceptible to aminoglycosides and ciprofloxacin, although resistant isolates may emerge in the course of chronic infections.
Publisher
American Society for Microbiology
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